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Mostrando postagens de maio, 2018

Plant Biotechnology and Global Sustainability - Part 1 of 4

11.2 billion. According to Roser and Ortiz-Ospina (2013) will be the number of people with whom we will share our planet in 2100. This includes land, water, air and all other natural and non-natural resources. As the population grows, so does the global need for food and consequently industry, leading proportionally to an increase in pollution. Of the various types that exist, air pollution can affect a large part of the world's population, even if it does not live near its source, being able to be present in its daily life, implying for example the worsening of diseases. In 2012, it caused about 3 million premature deaths (World Health Organization, 2016). One of the main sources of air pollution at a global level is transports (Karagulian et al., 2015), which means that both the production of the fuel and the release of gases by the means of transport releases harmful compounds to the environment, such as Carbon Dioxide (Kahn et al., 2007). This gas is very problematic due t

Domestication of Wheat

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Another crop of the utmost importance, for the present and the future population, is wheat (Triticum spp.). There are two main types of ancient domesticated wheat: the Einkorn (Triticum monococcum ssp. monococcum) and the Emmer (T. turgidum ssp. dicoccum L). They got replaced by a tetraploid free-threshing wheat (Durum Wheat) that evolved from the Emmer Wheat, that throuhout time give place to the common wheat (Triticum aestivum) we know today (Faris, 2014). There are 3 main genes involved in this crop domestication: Br (Brittle rachis), Q (free-threshing), and Tg (Tenacious glume) (Figure 1). In natural conditions, the seed dispersal is crucial for the dissemination of the species, a characteristic defined by brittle rachis (Br), but, for human use is not an advantageous strategy, because it difficult the harvesting process. This phenotype is controlled by a gene present in the 2A or 3A and 3B (depending on the species) chromosome, so a mutation must have occurred there to give ri

Why is it important to study the metabolic pathways of plants ?

Knowledge of metabolic pathways and the analysis of differences between plants can be a great help in obtaining several products very useful for several areas related to the vegetal component of biology as is the case of Agriculture. One such case is the use of herbicides that block the synthesis of lipids at the level of ACCase (enzyme acetyl-CoA carboxylase) in grasses. This type of herbicide allows the absence of competition in the production of dicotyledons and some monocotyledons since these are not as affected as grasses. ACCase catalyzes the passage of acetyl-CoA to malonyl-CoA in the lipid synthesis pathway. And this selectivity is due to the fact that there are two types of ACCase enzymes. The eukaryotic ACCase, present in the cytoplasm, and the prokaryotic ACCase, present in the chloroplast. Since the synthesis of fatty acids in dicots occurs in chloroplasts and the form that is inhibited by the herbicide is the eukaryotic form, the synthesis of lipids in the dicotyledon

Secondary metabolites of Plants

Secondary metabolites are chemical compounds produced only by certain organisms (mostly plant) and in certain circumstances (each species / genus produces a set of metabolites of its own). Having a very important role in the well-being of the organism that produces it, for example, the level of biotic stresses for protection against herbivores and the abiotic level in response to environmental changes. Among the various types of secondary metabolites are, for example, those containing nitrogen in the ring, such as Alkaloids; those composed mainly of carbon and hydrogen, such as terpenoids; and the phenolic compounds, which contain at least one aromatic ring and a hydroxyl group.  Alkaloids  can be derived from a variety of sources, such as lysine, ornithine, tyrosine, and tryptophan. Depending on the origin, several types of alkaloids are formed: Quinoleic alkaloids such as quinine, Pyridine alkaloids like nicotine, and isoquinoleic alkaloids such as morphine.  Terpenoid

How can the secondary metabolites help the plant that produce them ?

Plants face huge daily risks to their existence. These range from large animals and herbivorous insects to fungi and microbes; and abiotically can be debated with drought and excess solar radiation for example. The secondary metabolites may exert several roles in the host plant, the defense metabolism being unquestionable. The defense metabolites may be stored in the plant in their inactive form (phytoerycipins) or, they are only produced when the plant is attacked (phytoalexins). Among the 3 main categories of secondary metabolites are highlighted some compounds and their function. From the Terpenos family, Monoterpenoids and Sesquiterpenoids are the main constituents of essential oils that can be toxic to insects and also serve as protection against fungal and bacterial attacks. For example, pine trees have alpha and beta pinellas that function as insect repellents; also Chrysanthemum Pyrethrins work with insect neurotoxins. Tannins and Anthocyanins are examples of Phenolic Compo

Pharmacological effects of ginseng in the immune system

The pharmacological effects of ginseng  are mostly used therapeutically, ie, metabolites (such as ginsenosides) that affect a living system in order to treat a certain condition. There are several applications of ginsenosides in treatments of diseases and human conditions. Among them are anticancer effects, antidiabetics, antioxidants, among many others.  Here are some areas where ginseng exerts its positive effects: Immunity system Also certain activities of the immune system benefit from the presence of pharmacological compounds of ginseng (Kim et al ., 2013), such as phagocytic, antibacterial and antiviral. Macrophages increase phagocytic activity and nitric oxide production (NO), produced to destroy phagocytized organisms / compounds, in the presence of ginseng extracts (Shin et al ., 2002). Some Ginseng polysaccharides, such as PG-F2 and PG-HMV, are able to prevent the infectious process by microbes by disrupting their cell adhesion process (Lee et al ., 2009)

Pharmacological effects of ginseng on Cancer

The pharmacological effects of ginseng  are mostly used therapeutically, ie, metabolites (such as ginsenosides) that affect a living system in order to treat a certain condition. There are several applications of ginsenosides in treatments of diseases and human conditions. Among them are anticancer effects, antidiabetics, antioxidants, among many others.  Here are some areas where ginseng exerts its positive effects: Cancer There are 3 basic advantages inherent in ginseng regarding cancer and its treatment. Ginseng helps prevent cancer, the growth of cancer cells, inhibits the creation of metastases and reduces the side effects of chemotherapy. Prevention of Cancer and Inhibition of Growth of Cancer Cells - It has been found that ginsenosides Rg3 and Rg5 demonstrate the greatest preventative ability relative to cancer cells. One explanation for this is that attenuation of lipid peroxidation and the ability to scavenge super oxide. It has also been shown that t

Pharmacological effects of Ginseng in the Liver and Diabetes

The pharmacological effects of ginseng are mostly used therapeutically, ie, metabolites (such as ginsenosides) that affect a living system in order to treat a certain condition. There are several applications of ginsenosides in treatments of diseases and human conditions. Among them are anticancer effects, antidiabetics, antioxidants, among many others.  Here are some areas where ginseng exerts its positive effects: Liver In this organ ginseng has impacts in the fight against diseases caused by hepatotoxic agents and hepatitis. In the first case, the simple use of ginseng extracts with dietary honey can exert a synergetic effect to combat this type of diseases. The use of high doses of Paracetamol can lead to liver damage due to the effect of cytochrome P450 2E1 on acetaminophen (paracetamol), which converts it to N-acetyl-p-benzoquinone (hepatotoxin). Ginseng exerts a hepatoprotective effect due to Rg3 that inhibits the cytochrome in question (El Denshary et al .

Domestication of Citrines

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A study on citrines investigated the centre of origin of citrus species. Analyzing hybrids and admixed genomes they could find information on the domestication of mandarin, sweet oranges and other citrus. Wu et al . (2018) analyzed 60 accessions from diverse citrus germplasms and studied their relationship by performing whole genome sequencings. They found that mandarins contain pummelo admixture. The process of admixture is the result of interbreeding lineages from distinct sources and further introduction within a population (Keller and Taylor, 2010). This admixture was found to be corelated with fruit size and acidity, suggesting pummelo introgression as taking part in the selection of palatable mandarins during domestication, which requires reduced acidity (Baldwin, 1993). Palatable mandarins demonstrated pummelo admixture in part of the genome, opposite to acidic mandarins, proving the previous statement since, in mandarins, palatability is linked to pummelo introgression at a lo

Domestication of Tomato

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Solanum lycopersicum is the tomato species that we are most familiar with and that has been domesticated (Tanksley, 2004; Koenig et al., 2013). The exact domestication site of tomato is unknown but is thought to be placed in Mexico or Peru (Bai and Lindhout, 2007; Bergougnoux, 2014). On the other hand, what is known is that his domestication was a two-step process (Fig. 1) (Lin et al., 2014; Blanca et al., 2015).  These two steps determine the domestication and improvement sweeps, that consist in the increase of the frequency of a certain allele and the hitchhiking genes nearby, in the population (Schaffner and Sabeti, 2008; Lin et al., 2014).  The main phenotypic changes are the fruit size and shape and the seed size and weight (Bai and Lindhout, 2007). 8 There are 5 QTL (fw1.1, fw5.2, fw7.2, fw12.1 and lcn12.1) related to the first step of domestication involved in the increase of the fruit size and another 13 QTL (fw2.1, fw2.2, fw2.3, lcn2.1, lcn2.2, fw3.2, lcn3.1, fw9.1, f

Domestication of Rice

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Oryza sativa as it exists today underwent domestication by a series of occurrences of hybridization and introgression between ancient gene pools and inbred lines (Kovach et al., 2007) . 13 domestication traits were associated with 76 QTLs (Lai et al., 2003), some of the genes that contribute to these traits are BH4 for seed pigment, SH4-1 and qSH1 for seed shattering, SD1 for dwarfism and BADH2 for fragrance (Meyer and Purugganan, 2013). One of the most important case studies is the elimination of seed shattering in rice, which consists of retaining the seeds on the straws to facilitate harvest and increase production yield (fig. 1). Wild rice (O. rufipogon Griff.) was domesticated to O. sativa and the genes shattering4 and qSH1, responsible for the abscission layer formation, were selected a non-shattering allele for them (Kovach et al., 2007). The shattering QTL, qSH1, was identified from an intra-specific japonica x indica cross (Konishi et al., 2006). Studies show that a SNP can c

Crop domestication processes

In crop domestication two processes can occur: convergent phenotype evolution that represents the appearance of the same traits in independent organisms, for example different cereal crops and seed dormancy (sometimes not involving the same loci) (Gross and Olsen, 2010); and parallel phenotype evolution , that, like the convergent type there is an appearance of the same trait but in related lineages (Gross and Olsen, 2010). Both processes result either from an unconscious selection, that may be the initial and main source of domestication syndrome, a natural selection resulted from human cultivation; or as opposed, a conscious selection by cultivation and propagation of plants with favored phenotypes (Meyer and Purugganan, 2013; Ross-Ibarra et al., 2007). Another process involved in crop domestication could be polyploidy (genome duplication). Many species known as diploid are paleopolyploids, a study made by Van der Hoeven et al. (2002) indicates this event for several crops, am